Fig. 5. Preventing ER accumulation during fertilization. Unfertilized eggs were injected with Ca²⁺-Green-1-dextran and treated with 2 µg ml^–1 cytochalasin B or 2 µM nocodazole or a combination of both, and fertilized. (Ai) The pattern of Ca²⁺ oscillations in an egg treated with nocadazole alone; n=8. (Aii) The pattern of Ca²⁺ oscillations in an egg treated with cytochalasin B alone; n=9. (Bi) The pattern of Ca²⁺ oscillations in an egg treated with a combination of cytochalasin B (2 µg ml^–1) and nocodazole (2 µM). (Bii) All of the Ca²⁺ waves originated at the same site in the egg (boxed). This boxed area is shown enlarged and contains the round sperm with condensed DNA (circled area). The black asterisk shows the elongated form of non-incorporated sperm DNA, which remains attached to the outer surface of the egg. Temperature 19°C, n=18 replicates, 5 animals.