Fig. 6. Ca2+-Green-1-dextran was injected into unfertilized eggs. Subsequently, eggs were treated with cytochalasin B and nocodazole for 30 minutes and then injected with aSE. Thereafter, all Ca2+ waves originated at the cortex of the egg (A,B,C). Arrowheads mark the origin of three separate Ca2+ waves within the same egg. Temperature 14°C, n=6 replicates, 3 animals.