Fig. 8. Localization of megalin and MegBP in BN16 cells. BN16 cells were transiently transfected with the EGFP-MegBP expression constructs. Four hours later, the cells were fixed and incubated with a goat anti-rabbit megalin antibody followed by Cy5-labeled rabbit anti-goat IgG. Subcellular localization of megalin (A) and EGFP-MegBP (B) was detected by confocal fluorescence microscopy. Micrographs in A and B were merged to produce panel C. Colocalization of both proteins is indicated in yellow.