Fig. 3. Expression of 480-Cbl does not affect PDGF receptor activation or signaling to MAP kinase and PI 3-kinase. Wild-type, c-Cbl- and 480-Cbl expressing NIH 3T3 cells were lysed following serum starvation or after 1 and 5 minutes of PDGF activation as indicated. Aliquots of the lysates were subjected to SDS-PAGE and western blotting with anti-phosphotyrosine (A), anti-phospho-Shc (Tyr317) and anti-Shc (B), anti-phospho-MAP kinase (Thr202/Tyr204) and anti-MAPK (C) and anti-phospho-Akt (Ser473) and anti-Akt (D), as indicated.