Fig. 6. Effect of 480-Cbl expression on PDGF-mediated activation of Src. Wild-type, c-Cbl- and 480-Cbl-expressing NIH 3T3 cells were lysed following serum starvation or after 1 and 5 minutes of treatment with PDGF. Aliquots of the lysates were directly subjected to SDS-PAGE and probed by western blotting with anti-phospho-Shc (Y239) and anti-Shc (A) or anti-phospho-Stat3 (Y704) and anti-Stat3 (B) as indicated.