Fig. 5. CycB2-HA localization in cells treated with roscovitine, APM, taxol and MG-132. (A) Cells were treated in G2 phase with 100 µM roscovitine and samples were taken after five hours incubation; arrows indicate a prophase-arrested cell. Immunostaining of CycB2-HA expressing cells with the anti HA-antibody is shown in the left-hand panels and DAPI-staining of the chromatin in the right-hand panels. (B) Cells were treated in G2 phase with 10 µM amiprophos methyl (APM) (upper panels) or 50 µM taxol (lower panels) and samples were taken after 5 hours incubation; arrows indicate metaphase-arrested cells. (C) Cells were treated in G2-phase with 100 µM MG 132 and samples were taken after 5 hours. The arrows indicate a cell arrested in mitosis. Scale bars: 10 µm.