Fig. 6. Cyclin B1-GFP but not cyclin B2-GFP is stabilized in living cells arrested
in mitosis by activation of the spindle checkpoint or by inhibition of the
proteosome. (A) Visualization of GFP fluorescence in living cells expressing
cyclin B1-GFP (left panels) or cyclin B2-GFP (right panels) after treatment
with propyzamide. Arrows indicate cells arrested in mitosis. (B) Visualization
of GFP fluorescence of Cyclin B1-GFP (left panels) or cyclin B2-GFP (right
panels) in living cells after treatment with epoxomicin. Arrows indicate cells
arrested in mitosis. Upper panels, Fluorescent images; lower panels, DIC
images. Scale bars: 10 µm.