Fig. 7. Protein levels of ectopically expressed CycB2-HA and endogenous cyclin B1 in synchronized CycB2-HA-2 cells and after treatment with propyzamide, MG 132, epoxomicin or lactacystin. (A) Immunodetection of CycB2-HA protein with an anti HA-antibody in protein samples prepared from non induced control cells or in samples prepared at 2 hour intervals from 7 to 15 hours after aphidicolin release and at 15 hours in cells that were treated in late G2-phase with propyzamide (P), epoxomicin (E), lactacystin (L) or MG132 (Mg). (B) Immunodetection of endogenous CycB1 protein with an anti N-terminal CycB1;1 peptide antibody in the same synchronized samples as described in A, and in samples treated with propyzamide (P) epoxomicin (E) and lactacystin (L).