Fig. 8. Root regeneration is inhibited by ectopic expression of CycB2-HA, which correlates with shortening of the G2 phase in leaf cells. (A) Leaf disks from line CycB2-HA-2 were incubated on root-inducing medium containing 0.5 mg/l auxin (NAA) and 0.1 mg/l cytokinin (BAP) in the presence (+) or absence (-) of 0.1 mg/l Cl-tetracycline and pictures of regenerates were taken after 3 weeks. (B) Flow cytometry measurements of G1 and G2 DNA contents in isolated nuclei from leaves treated as in A and incubated for 3 days.