Fig. 2. Differential binding of Snail and Slug to the E-pal element. Recombinant GST-Slug (left panel) and GST-Snail (right panel) proteins were incubated at the indicated concentrations (50-500 ng) with the ³²P-labeled wild-type E-pal probe in the presence or absence of 1,000-fold molar excess of the wild-type or mutant cold oligonucleotides or in the presence of 5 µg of anti-Slug monoclonal antibody (left panel) or anti-Snail polyclonal antibody (right panel). The different retarded complexes are indicated by arrows and the supershifted complexes by arrowheads. Wild-type E-pal and mE-pal oligonucleotides are as shown in Fig. 1.