Fig. 5. Stable transfection of Slug into MDCK cells induces epithelial to mesenchymal conversion concomitantly with the loss of epithelial markers and the gain of mesenchymal markers. (a-f) Phase-contrast images of living, subconfluent cultures of a mock-transfected clone (a), four Slug-transfected clones (b,c,d,e) and Snail-transfected cells (f). (g-x) Immunofluorescent images of the indicated cell lines showing the localization and organization of E-cadherin (g-l), fibronectin (m-r) and vimentin (s-x). See the loss of E-cadherin stain and the increased expression and fibrous organization of fibronectin and vimentin in the Slug and Snail transfectants. Slu2 and Slu3 represent independent clones; Slu1I and Slu1 III represent two subclones isolated from an original Slu1 clone. Bars, 40 µm (a-f); 20 µm (g-x).