Fig. 2. Immunolocalization of ND10-associated proteins after Cd²⁺ exposure of HEp-2 cells. Proteins shown are indicated at the upper left and right of each panel in the color used. For control images, see Fig. 1A-C. (A) Daxx, SC35 and coilin. Daxx is totally dispersed, whereas SC35 and coilin are apparently distributed as in control cells. (B) The nuclear envelope and HSF1. Daxx is dispersed and HSF1 is aggregated, whereas the nuclear envelope and nucleoli appear intact. (C) Most but not all cells exposed to 50 µM Cd²⁺ for 4 hours show Daxx and PML dispersed. (D) Same as A but stained for SUMO and Sp100. SUMO is dispersed but most cells retain a few Sp100 aggregates. (E) Cells treated with SB203580 for 3 hours and exposed to 50 µM Cd²⁺ for 5 hours and labeled for Sp100 and SUMO-1. ND10 are not dispersed. (F) HEp-2 cells exposed to PD98059 for 5 hours and labeled for Sp100 and SUMO-1. ND10 are not dispersed.