Fig. 2. Distribution of Lyn, CD55, TfR and FcRII in density gradient fractions. U937 cells unexposed to inhibitors (controls, Ctrl) or preincubated with 1 mM HMA, 0.3 mM BPA or 5 mM CDX were lysed and fractionated on a density gradient in the presence of 0.2% TX-100. Fractions were immunoblotted for the presence of Lyn, CD55 and TfR (A) or biotin-labeled IV.3 anti-FcRII reflecting the receptor distribution (B). In B, the distribution of cross-linked (+) and non-crosslinked (-) FcRII is shown; in A, proteins of fractions derived prior to FcRII labeling are demonstrated. Plots display the results of densitomeric analysis of the blots shown above. The estimated amounts of an individual protein in fractions are expressed as a percentage of this total protein content in the whole gradient. Symbols used for visualization of various experimental conditions in plots of (A) and (B) are shown at the bottom of panel (B). The results are representative of three to five separate experiments.