Fig. 2. RAD52-independent homologous repair of a dicentric chromosome in cells containing the cdc5-ad allele requires RAD1 and RAD9. (A) Southern analysis of CEN3-GALCEN recombination in a cdc5-ad strain following activation of a dicentric chromosome. (B) CEN3-GALCEN recombination also occurs in a cdc5-ad rad52 strain following activation of a dicentric chromosome but this recombination product is not detected in a cdc5-ad rad1 rad52 double deletion strain (C) or in a cdc5-ad rad9 rad52 strain (D). The arrow indicates the position of the original band and the arrowhead indicates the recombinant band. The numbers below the lanes refer to time of sample collection, in hours, following transfer of strains from galactose to glucose. The positions of molecular weight markers (kb) are indicated.