Fig. 1. Immunofluorescence microscopy of cultured Jurkat T cells during Fas-mediated apoptosis. Cells grown in suspension were untreated (A-E) or treated (F-J) with Fas antibody for 120 minutes, fixed and double-stained with NuMA (A and F) and lamin B (B and G) primary antibodies and FITC-conjugated rabbit-anti-mouse (A and F) and TRITC-conjugated rabbit-anti-goat (B and G) secondary antibodies. Samples were counterstained for DNA with Hoechst (D and I). Note the uneven condensed distribution of NuMA and wrinkled distribution of lamin B in early apoptotic cell (arrow) and the presence of NuMA and lamin B around fragmented nuclei in apoptotic bodies (arrowheads). Bar, 5 µm.