Fig. 11. NuMA and lamins are not degraded in MCF-7 cells during staurosporine-induced apoptosis. Cells grown on a monolayer were untreated (lane 1) or treated (lane 2) with 2 µM staurosporine for 12 hours and prepared for SDS-PAGE. Identically loaded gels were run and immunoblotted with NuMA, lamin A/C, lamin B, PARP-1, caspase-8 or caspase-7 antibody. Note the partial cleavage of PARP-1, procaspase-8 and disappearance of pro-caspase-7 in staurosporine-treated cells. NuMA and lamins are not cleaved during the apoptosis.