Fig. 2. The cleavage of NuMA, lamin B and poly(ADP-ribose) polymerase-1 (PARP-1) during Fas-mediated apoptosis in Jurkat T cells. Jurkat T cells were treated with Fas antibody and prepared for SDS-PAGE at different time points as indicated. Identically loaded gels were run and immunoblotted with NuMA, lamin A/C, lamin B or PARP-1 antibody. Arrowheads indicate the cleavage products of NuMA, lamin B and PARP-1. Lamin A/C antibody does not detect lamin A or C in Jurkat T cells. Fas-antibody- and PD 98059-treated and untreated HeLa cells were used as controls (both 36 hours, see later).