Fig. 8. The amount of apoptotic, atypical apoptotic and NuMA negative nuclei in the presence of caspase inhibitors. HeLa cells grown on glass coverslips were treated with Fas antibody and PD 98059 for 24 hours in the presence or absence of 100 µM z-DEVD-FMK, z-VEID-FMK or z-IETD-FMK and processed for immunofluorescence microscopy analyses. 800-1200 adherent cells in randomly selected areas were counted for each coverslip. The data represent mean values and standard deviations from three separate experiments. Note the correlation between the numbers of atypical apoptotic and NuMA negative nuclei.