Fig. 1. Schematic representation of the hRUL138 ORF and protein. (A) cDNAs identified in this study. The line on the top represents the complete hRUL138 ORF, the bars below indicate cDNA sequences. Numbers are nt positions relative to the hRUL138 ORF. Non-translated regions are depicted by hatched bars and regions encoding identical amino-acid sequences as in hRUL138 by dark grey bars. Light grey stippled bars correspond to identical nucleotide sequences which are, however, out of frame owing to small, splicing-related deletions (shown as ) that lead to early translational stops (^*); (A)_n denotes a poly-A tail. White bars indicate introns that have not been spliced out in the corresponding cDNAs. NIII is the cDNA initially isolated by Northwestern screening, the other cDNAs were identified by molecular hybridization using an NIII derived DNA probe (black line above NIII). (B) Predicted motifs within the hRUL138 primary sequence. Numbers are amino acid positions. The lysine-rich (K-rich) motif and the RING-H2 domain are indicated by black boxes; the RING-H2 sequence is shown below with the conserved Cys and His residues highlighted in bold face. The prediction for the coiled-coil motif is strong between amino acids 794 to 852 and extends with weaker scores as indicated by the hatched bars. The weakly and variably predicted putative transmembrane regions are denoted by TM?