Fig. 8. Intracellular localization of hRUL138. Huh7 cells were transfected with expression constructs for the GFP fusion proteins indicated on the top of each panel. For colocalization studies, the indicated hRUL-GFP construct was co-transfected with plasmid pRFP2-ER, encoding an ER-targeted red fluorescent protein. Unfixed cells were observed by confocal laser scanning microscopy, except for the TOTO-3 counterstaining in panel B; there GFP was detected indirectly using mouse anti-GFP antibodies and a fluorescently labeled anti-mouse IgG antibody. (A) Localized distribution of hRUL138-GFP versus even distribution of GFP alone. (B) Extranuclear staining by hRUL1-1128-GFP versus blue nuclear staining by TOTO-3. (C) Coexpression of hRUL1-1128-GFP and RFP2-ER. (D) Coexpression of hRUL510-878-GFP and RFP2-ER.