Fig. 2. [³⁵S]Met/Cys incorporation into A. polypoides tissue fragments after exposure to a temperature rise. Freshly cut A. polypoides fragments were incubated in SW at 24°C for 3 hours with 20 µM EGTA-AM, or with 10 µM 8-Br-cADPR, or without any addition (see key). Tissue pieces were then transferred into fresh SW at 16°C and [³⁵S]Met/Cys was added to the tubes for the last 6 hours before harvest. Radioactivity incorporation, determined as described in Materials and Methods, is expressed as variations relative to respective controls, i.e. fragments incubated at 16°C throughout the experiment. x-axis numbers indicate the total length of the incubations. Results are the mean±s.d. of four experiments, performed on different animals.