Fig. 4. Effect of inhibitors of the temperature-signaling cascade in A.
polypoides on temperature- and ABA-induced short-term stimulation of
O2 consumption and filtration rate. O2 consumption and
dye filtration were recorded for 2 hours before (control) and after exposure
of A. polypoides fragments to heat stress, ABA or 8-Br-cAMP. EGTA-AM
(15 µM) and 8-Br-cADPR (10 µM) were added 30 minutes before ABA (20
µM). Bupivacaine (BUPI; 1 mM) and GdCl3 (50 µM) were added
during heat stress (24°C for 30 minutes). The calculated slope of the
linear regression curve (R
0.98) is compared with that of the respective
control, i.e. the same fragment prior to stress induction or addition of
chemicals. Data are the mean±s.d. of five experiments.