Fig. 3. PKB/Akt is phosphorylated in mitosis and associates with the centrosome. (A) HeLa whole cell lysates probed for total PKB and phospho-PKB (T308). S, serum-stimulated HeLa cells; U, unsynchronised HeLa cells; M, mitotic HeLa cells. (B) Immunofluorescence microscopy of HeLa cells. Cells were fixed and stained with anti-PKB antibody. DNA was counterstained with DAPI. (C) Localisation of Phospho-PKB (Thr308) in HeLa cells. Cells were fixed using methanol (-20°C). Bar, 10 µm.