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Fig. 5. Inhibition of GSK-3 does not affect centrosome separation. Actively cycling HeLa cells were treated with the GSK-3 inhibitor SB-415286 for 90 minutes before fixation with methanol (-20°C). Coverslips were stained with antibodies to {gamma}-tubulin and with DAPI to visualise DNA. In cells possessing mono-oriented chromosomes {gamma}-tubulin staining is seen as two discrete dots, one at either side of the metaphase plate. Identical results were obtained using SB-216763.