Fig. 6. Inhibition of GSK-3 dramatically reduces chromosome dynamics in mitosis. HeLa cells stably expressing Histone 2B-GFP were imaged at 37°C at 3 second intervals for 30 minutes in the absence (A) or presence (B) of the GSK-3 inhibitor, SB-415286. (A) The arrow in the image at 300 seconds marks the position of a chromosome that undergoes significant movement. This movement is shown enlarged in panels 279-458s. (B) The arrow in the image at 300 seconds marks the position of a chromosome that does not undergo significant movement. This is shown enlarged in panels 312-492s. Bars, 10 µm. QuickTime^TM movies of time-lapse sequence shown in A and B are available online as Movies 1 and 2, respectively (http://jcs.biologists.org/supplemental).