Fig. 7. Inhibition of GSK-3 results in inhibition of chromosome oscillation and congression. Manual particle tracking was used to quantitate the time-lapse data from Fig. 6. Tracks shown in green in Fig. 7 represent movements of the chromosomes highlighted in the zoomed images in Fig. 6C. Starting points of two dynamic chromosomes for control (A) or inhibitor treated cells (B) are shown as white spots with their subsequent trajectories during the time-lapse sequence shown as red or green overlays. (C,D) The total distance moved of each of these chromosomes is shown as a function of time. Bars, 10 µm. (E-F) Tracking record of mono-oriented chromosomes. (E) In an untreated cell, the chromosome performs several rapid, reversible movements before moving into the metaphase plate. (F) A cell treated with SB-415286. The unaligned chromosome fails to show similar oscillations. Even after a further 30 minutes this chromosome does not approach the metaphase plate (D.J.S., unpublished) (for the movies of these chromosomes, see http://jcs.biologists.org/supplemental).