Fig. 2. Amino acids 1-63 of AE1-4 can direct a cytoplasmic tailless mutant of the F_c receptor to the basolateral membrane of MDCK cells. Confluent MDCK cells stably expressing F_c^- (A), F_c1-63 (B), F_c1-63Y44A (C), F_c1-63Y47A (D) or F_c1-63Y44A, Y47A (E) were fixed and incubated with the rat monoclonal antibody specific for the F_c receptor and phalloidin-FITC. The cells were then washed and incubated with donkey anti-rat IgG conjugated to lissamine, and the distribution of fluorescently labeled proteins was visualized using a Zeiss LSM510 confocal microscope. The 0.5 µm xy image in each panel is near the center (B, C and D) or near the apical surface (A and E) of the cells. Regions that are yellow indicate significant overlap in the distribution of the chimera and actin. The black arrowhead next to each panel marks the position of the basal membrane in the xz image of the transfected cells. The bar in each xy image is 10 µm.