Fig. 8. F_c1-63 and F_c38-63Y47A colocalize with phalloidin-stained stress fibers in subconfluent MDCK cells. Subconfluent MDCK cells stably expressing F_c1-63 (A-C) or F_c38-63Y47A (D-F) were fixed, permeabilized and incubated with the F_c-receptor-specific antibody (A,D) and phalloidin conjugated to FITC (B,E). The cells were then washed and incubated with donkey anti-rat IgG conjugated to lissamine. Following washing, the localization of fluorescently labeled polypeptides was visualized on a Zeiss Axiophot microscope. The merged images showing the overlap of these chimeras with phalloidin-stained microfilaments are shown in C and F. Bars, 10 µm.