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Fig. 2. Expression of EGFP/hSP-C, pcDNA3/hSP-C and HA/hSP-C in transfected A549 cells. Plasmids containing cDNA for EGFP/hSP-C (A), pcDNA3/hSP-C (B), and HA/hSP-C (C) were introduced into A549 cells by CaPO4 precipitation. 48 hours after transfection, cells were fixed and/or stained with polyclonal {alpha}-NPROSP-C and Texas-Red-conjugated goat anti-rabbit serum or monoclonal anti-HA plus Texas-Red-conjugated goat anti-mouse IgG. The fluorescence images were acquired using High Q FITC filter package for EGFP/hSP-C (A) or a High Q Texas Red filter package for NPROSP-C (B) and HA staining (C). Corresponding phase images appear below each fluorescence panel.