Fig. 5. Ubiquitinated EGFP/hSP-C^Exon4 is associated with the MOTC. A549 cells grown on coverslips were transfected with EGFP/hSP-C^Exon4 using CaPO₄. Forty-eight hours after introduction of plasmid DNA, cells were fixed and staining was performed using monoclonal antiserum against either ubiquitin (A-C) or -tubulin (1:100) (D-F) and visualized with secondary goat antimouse-IgG—Texas-Red. Two channel fluorescence images were acquired for EGFP (A,D) and Texas Red (B,E).