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Fig. 7. hSP-C{Delta}Exon4 alters trafficking of wild-type proSP-C. A549 cells were co-transfected with 5 µg of HA/hSP-C1-197 plasmid plus 5 µg of either EGFP/hSP-C1-197 (A-C) or EGFP/hSP-C{Delta}Exon4 (D-F) plasmids using CaPO4. Cotransfected cells fixed 48 hours after transfection, permeabilized and stained with anti-HA using Texas-Red-conjugated secondary antibody were subjected to fluorescence microscopy for EGFP (A,D) and {alpha}-HA (Texas Red) staining (B,E). EGFP- and HA-tagged hSP-C1-197 colocalize in the same cytoplasmic vesicles. In contrast, EGFP/hSP-C{Delta}Exon4 induced aggregation of HA/hSP-C1-197 in perinuclear compartments.