Fig. 8. 4-Phenylbutyric acid blocks accumulation of EGFP/hSP-C^Exon4 in aggresomes. A549 cells grown on coverslips were transfected with 5 µg EGFP/hSP-C^Exon4 using CaPO₄. At the time of transfection, cells were treated with either 0, 1 mM, or 5 mM Na 4-phenylbutyrate as indicated. Forty-eight hours after introduction of plasmid DNA, cells were fixed and fluorescence images for EGFP expression were acquired.