Fig. 1. C6-conditioned media protects against hypoxia-induced permeability changes in BBMEC monolayers. BBMEC monolayers were incubated for three days in C6-CM or RA-CM and then subjected to 24 hours of normoxia or hypoxic stress (1% O₂). Under normoxic conditions, neither C6-CM nor RA-CM has any effect on BBMEC monolayer permeability. However, after 24 hours of hypoxia, there is a significant increase in permeability in all three treatment groups. This increase in permeability after hypoxic stress is partially blocked by co-culture with C6-CM. ^***P<0.001 versus comparable normoxic treatment group, ^###P<0.001 versus hypoxic MEM/F12, ^{^}P<0.05 versus hypoxic RA-CM, n=16.