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Fig. 2. (A) The genomic structure of forC and the forC disruption construct. (B) Agarose gel electrophoreses of the forC locus obtained by genomic PCR from wild-type (Ax2) and {Delta}forC cells. Amplification of wild-type genomic forC locus yielded a 3.6 kb product; amplification of the forC knocked-out locus yielded a 2.4 kb product.