Fig. 4. Expression of forC at each stage during development. Total RNA was prepared at several time points during development, and RT-PCR was carried out using primers designed to amplify a 983 bp fragment that included a site from which an intron was excised. The time after the onset of starvation is indicated below each picture, and the status at each developmental stage is illustrated above the picture. 330 bp H7 gene fragment was amplified as an internal control (Zinda and Singleton, 1998).