Fig. 6. Intracellular localization of GFP-ForC. (A) Live observation of forC cells expressing GFP-ForC in MES buffer. GFP-ForC was diffusely distributed in the cytoplasm. (B) forC cells expressing GFP-ForC were fixed and stained with rhodamine-phalloidin. The fluorescent signals were recorded separately from the GFP and rhodamine channels by using a CCD camera, and then pseudocolored and merged. GFP-ForC localized at the crowns (a,b), which are rich in F-actin (a', b' and c'), while GFP alone had no distinct localization (c). GFP-ForC co-localizated with F-actin at crowns were depicted in yellow in merged pictures (a'',b''). No yellow region is seen in the merged images of cells expressing GFP alone (c''). (C) Localization of GFP-ForC at the crowns in live cells compressed by agarose overlay. Arrows indicate GFP-ForC fluorescence.