Fig. 8. Intracellular localization of GFP-ForC-1-323 during macropinocytosis, phagocytosis, and when touching a neighboring cell. Images were taken every 6 seconds using confocal microscopy. (A) Arrows indicate a typical crown during macropinocytosis. GFP-ForC-1-323 stays at the leading edge of the ruffling membrane until it eventually disappears. (B) Arrows indicate a phagocytotic cup engulfing a yeast cell. The yeast cells being engulfed and those already taken up by the Dictyostelium cell are visible due to their autofluorescence. (C) Arrows indicate the site at which a cell touches a neighboring cell.