Fig. 6. CNF-1 does not dramatically influence AJ protein localization in the lateral membrane. E-cadherin and ß-catenin were immunolocalized in confluent T84 monolayers exposed basolaterally to CNF-1 (1 nM, 48 hours) or vehicle. In control monolayers, both E-cadherin (a) and ß-catenin (c) were visualized en face in characteristic ring formations in the AJ. Basolateral exposure to CNF-1 did not visibly alter the ring pattern of E-cadherin (b), although minor thickening of ß-catenin staining intensity was observed at cell-cell borders (d). Total protein levels of E-cadherin (e) and ß-catenin (f) were unaffected by CNF-1 treatment (lanes 1-3 control, 4-6 CNF-1). Bar10 µm.