Fig. 4. ß-COP labeling of wound-edge NIH 3T3 cells microinjected with different pEGFP-mDia1 expression constructs. (A) The main domains contained in the four mDia1 constructs used in this study are shown. Full-length mDia1 contains the following domains from N- to C-terminus: N3, FH1 and FH2. All constructs are tagged with GFP as indicated. (B) The microinjected cells expressing the GFP-fusion protein are shown as indicated (top panels): mDia-deltaN3 (left), mDia F2 (middle) and mDia-deltaN3KA3 (right). Cells were fixed and stained for the Golgi apparatus with the anti-ß-COP antibody (bottom panels). Bar, 40 µm.