Fig. 7. Motility of the microinjected wound-edge NIH 3T3 cells within the cell sheet during wound closure. Cells were microinjected with pEGFP-expression vectors encoding mDia-deltaN3 (left panels), mDia F2 (middle panels) and mDia-deltaN3KA3 (right panels). Cells were fixed 4 hours after microinjection and labeled for actin. (A) GFP-mDia-deltaN3-, GFP-mDia F2- and GFP-mDia-deltaN3KA3-expressing cells are shown in the top panels (left to right panels, respectively, as indicated). Actin labeling is shown under the corresponding top panel and merges of the two top panels are shown at the bottom of the corresponding column. Cells expressing mDia-deltaN3 and mDia F2 were left behind the wound edge (left and middle columns). Cells expressing mDia-deltaN3KA3 were motile as they persisted at the margin of the closing wound (right column). (B) The table includes the percentage of migrating cells for each expression construct microinjected in wound-edge cells and the number (n) of microinjected cells observed. Quantification was performed from three separate experiments for each construct. Bar, 40 um.