Fig. 1. A nonsense mutation in a katanin p60 ortholog gene (AtKSS) is responsible for the lue1 phenotype. (A) Rescue of lue1 phenotype with the C3300-AtKSS transgene. (B) Wild-type (top) and lue1 (bottom) bioimaging. (C) lue1 bioimaging. (D) lue1 C3300-AtKSS bioimaging. (B-D) Bright field image (left), LUC in vivo image (center), superimposition of bright field and LUC images (right). (E) RNA blot analysis of AtKSS mRNA accumulation in lue1, wild-type transgenic Col0 (WT), Ler and the GA-deficient ga1-1 mutant (top). GA treatment (50 µM GA₃) was applied to the ga1-1 mutant for 2 or 24 hours. Ethidium bromide staining of the nitrocellulose membrane after RNA blotting (rRNA, bottom).