Fig. 4. Lue1 exhibits disorganized CMF and CMT. (A,B) Polarizing microscopy of WT and lue1 CMF orientation in different cell types. (A) CMF orientation in single pitted vessel cells of WT and lue1 showing maximum birefringence for rotation angles relative to main growth axis of 45° and 0°, respectively. This indicates a transverse orientation of CMF compared with the main growth axis in WT, whereas the average CMF orientation in lue1 is 45°. Bars represent 5 µm. (B) Deviation from transverse orientation of CMF in WT and lue1 cells (n minimum=20). (C-F) Confocal microscopy of CMT organization in WT and lue1 cells. Bars represent 10 µm. The microtubule-decorating GFP-MAP4 reporter was introduced in lue1 by crossing and CMT organization assessed in segregating F₂ seedlings. WT epidermal root (C) and stomata (E) cells. Lue1 epidermal root (D) and stomata (F) cells.