Fig. 1. Production of nidogen-binding site constructs and controls. The site for
interaction between laminin-1 and nidogen-1 has been localized to LE module 4
of domain III of the laminin
1 chain
(Mayer et al., 1993). Two
different FLAG fusion proteins (indicated with flags) were constructed and
modified with the BM40 signal peptide to ensure secretion to the extracellular
space,
1III3-5 coding for LE modules 3-5 and
1V1-3 comprising
three LE modules of domain V.