Fig. 4. Immunoblot for laminin-1 and nidogen-1 in embryoid body total lysates. A rabbit polyclonal antibody against laminin-1 and nidogen-1 was used to detect these proteins in total lysates prepared from 12-day-old retinoic-acid-treated embryoid bodies in SDS. Equal loading and transfer to nitrocellulose were confirmed by Ponceau Red staining of the membrane (not shown).