Fig. 5. Comparison of diffusion coefficients obtained from incubation of differentiated F9-cell-derived embryoid bodies with rhodamine-labelled dextrans of 10 kDa and 70 kDa. Embryoid bodies stably expressing the constructs 1III3-5, 1V1-3 and a control clone were incubated with 10 µM dextrans of either size. Fluorescence intensities were measured inside the embryoid bodies after 5 minutes of diffusion. (A) Representative curves of single embryoid bodies (control and 1III3-5/A). incubated with 10 kDa and 70 kDa fluorescent dextrans. (B) From at least six traces of individual embryoid bodies, mean diffusion coefficients plus s.d. were calculated. Two independent experiments were performed with comparable results.