Fig. 6. Treatment with colchicine does not induce phosphorylation of p42/44 MAPK in neutrophils. Neutrophils were either incubated for 40 minutes at 37°C in medium or were exposed, after preincubation in medium for 10-35 minutes, for the times indicated to 1 nM fNLPNTL or to 10 µM colchicine (total incubation time: 40 minutes). Cells were precipitated with TCA and subjected to immunoblotting in order to visualize phosphorylated p42/44 MAPK. An immunoblot representative of four experiments is shown. Note, for the control and the samples incubated with colchicine, 1.8x10⁵ cells were applied per lane; for the sample incubated with fNLPNTL, 0.2x10⁶ cells were applied per lane.