Fig. 8. Colchicine treatment increases myosin light chain phosphorylation in neutrophils in a Y-27632-sensitive manner. Neutrophils were either incubated for 50 minutes at 37°C in medium or in 10 µM Y-27632 or were preincubated in the absence or presence of 10 µM Y-27632 for 30 minutes followed by a further incubation with 1 nM fNLPNTL for 5 minutes or with 10 µM colchicine for 20 minutes as indicated. Cells were precipitated with TCA and subjected to immunoblotting. (A) Immunoblot of a representative experiment (for cells exposed to colchicine, duplicates are shown). (B) Quantitative evaluation of A (mean±s.e.m. of five to six experiments; control with Y-27632: n=2).