JCS -- Sridharan et al. 116 (5): 823 Figure IG1

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 1. Relocalization of ${alpha}$ SpII ${Sigma}$ * to nuclear foci following treatment of normal cells with 8-MOP plus UVA light. The localization of ${alpha}$ SpII ${Sigma}$ * in the nucleus of undamaged normal human lymphoblastoid cells or lymphoblastoid cells treated with 8-MOP plus UVA light (6 kJ m^-2) was examined by immunofluorescence with purified monoclonal anti- ${alpha}$ -spectrin antibody. Counterstaining for the DNA-specific dye DAPI is shown in the middle panels. In the bottom panels, the fluorescent signal for DAPI was overlaid on that for anti- ${alpha}$ -spectrin. Cells were fixed for staining 15 hours after treatment with 8-MOP plus UVA.