Fig. 5. Failure of localization of SpII*, FANCA and XPF to nuclear foci in FA-A cells after treatment with 8-MOP plus UVA light. FA-A (HSC 72) lymphoblastoid cells were either undamaged or treated with 8-MOP plus UVA light (6 kJ m^-2) and localization of SpII*, FANCA and XPF in the nuclei examined 15 hours after treatment using immunofluorescence. Cells were in addition stained with the appropriate preimmune serum. (A) Dual staining was carried out using anti--spectrin (green) and anti-FANCA (red) antibodies. Fluorescent signals were not observed for FANCA. Only low levels of SpII* were observed in undamaged nuclei and a small number of SpII* foci were observed in the damaged nuclei. Merged signals show only staining for SpII*. (B) Similar analysis as in (A) was carried out using anti--spectrin (green) and anti-XPF (red) antibodies. Fluorescent signals for both proteins were merged. The yellow dots in the merged images indicate co-localization of both proteins. (C) Dual staining using anti-FANCA (green) and anti-XPF (red) was carried out. Only a few damage-induced foci were observed for XPF in the treated cells and none for FANCA. Merged signals show only staining for XPF.