Fig. 6. In corrected FA-A cells, SpII*, FANCA and XPF co-localize to discrete nuclear foci after DNA cross-link damage. FA-A (HSC 72) cells expressing the FANCA cDNA were either undamaged or treated with 8-MOP plus UVA (6 kJ m^-2) and examined for the presence of SpII*, FANCA or XPF in the nuclei. Cells were fixed 15 hours after treatment. (A) Immunofluorescence with anti--spectrin (green) and anti-FANCA (red) antibodies was examined. When the green and red signals were merged, the overlapping foci appeared yellow, indicating co-localization of SpII* and FANCA. (B) An analysis similar to that in (A) was carried out using anti--spectrin (green) and anti-XPF (red) antibodies. The fluorescent signals were merged and analyzed as above. (C) Dual staining with anti-FANCA (green) and anti-XPF (red) antibodies was also examined. Again, the fluorescent signals were merged and overlapping foci appear yellow.